Font size:
Print
Interplay Between High-Affinity DNA and Carbon Nanotubes
Context:
- In a new study, researchers from Pusan National University employed high-throughput screening methods to explore the relationship between DNA sequences and their binding affinity to carbon nanotubes.
- They focused on optimising the binding affinity and stability of these constructs through advanced sequence design and molecular dynamics simulations.
Single-walled carbon nanotubes (SWCNTs)
- They have garnered attention for their exceptional physical and chemical properties, making them promising candidates for applications in biotechnology and nanoelectronics.
- Challenges such as insolubility and toxicity have hindered their widespread use.
- To address these issues, researchers delved into the interplay between high-affinity DNA and SWCNTs.
Key Highlights:
- The researchers focused on improving the stability and safety of SWCNTs by functionalising them with optimal single-stranded DNA (ssDNA) sequences.
- They used molecular simulation to validate the binding affinity and machine-learning models to predict how ssDNA interacts with SWCNTs.
- The rigorous methodology involved iterative rounds of screening a diverse random 30-nucleotide ssDNA library to identify high-affinity sequences.
- Computational modelling, particularly molecular dynamics simulations, provided insights into the structural dynamics of the ssDNA-SWCNT complexes.
- The researchers developed a freely accessible online service that predicts the binding affinity of ssDNA sequences to SWCNTs.
Study Findings
- High-affinity 30-nt ssDNA sequences, rich in adenine and cytosine, showed superior binding strength.
- Stable intramolecular hydrogen bonds formed near the SWCNT surface, enhancing structural integrity.
- Machine-learning models accurately predicted binding affinities.
- Improved resistance to enzymatic degradation compared to free ssDNA.
Applications and Impact:
- High-affinity ssDNA-SWCNT constructs are suitable for long-term biological applications.
- Potential applications include cell or tissue-specific drug delivery systems and high-performance nano-electronic devices.
Future Prospects:
- The study advances the understanding of ssDNA and SWCNT interactions and offers practical applications in advanced technologies.
- Developing nanomaterials and devices with enhanced stability can drive innovation in nanoelectronics and biotechnology.
About Carbon Nanotubes:
- They are allotropic forms of elemental carbon, consisting of hexagonal sheets of single-layer hybridised carbon atoms rolled up in cylindrical form.
- A carbon nanotube has a diameter of a few nanometres and a length of several micrometres.
- Types of Carbon Nanotubes:
-
- Single-Walled Carbon Nanotubes (SWCNTs): They are composed of a single graphene layer rolled into a hollow cylinder. Often referred to as graphene nanotubes (GNTs).
- Multi-Walled Carbon Nanotubes (MWCNTs): They consist of multiple layers of graphene rolled into concentric tubes.
- Properties: High electrical conductivity, Mechanical strength, Stability, Chemical resistance, Thermal conductivity, and Load-bearing capacity.
